Question 1 (9 points) Consider the following piece of messenger RNA: 5´-AUGGAGUC

Question

Question 1 (9 points)
Consider the following piece of messenger RNA:

5´-AUGGAGUCGUUAAUUAAACCGGUGCGGAUCGUAUUUAGUCCCCAC-3´

(a) Draw both strands of the segment of DNA that this mRNA was transcribed from.
(b) State which of the DNA strands the RNA synthesizing enzyme used as template for the transcription process; i.e., which strand is referred to as the template/antisense strand?
(c) Which of the DNA strands is the sense/coding strand?
(d) Using the codon chart, give the amino acid sequence of the protein that would be produced by translation of the mRNA, assuming the ribosome moved along the mRNA from left to right.

Explanation / Answer

a) mRNA: 5`-AUGGAGUCGUUAAUUAAACCGGUGCGGAUCGUAUUUAGUCCCCAC-3´

DNA: 3` - TACCTCAGCAATTAATTTGGCCACGCCTAGCATAAATCAGGGGTG - 5`

DNA: 5` - ATGGAGCCGTTAATTAAACCGGTGCGGATCGTATTTAGTCCCCAC - 3`

b) The template or antisense starnd used is

DNA: 3` - TACCTCAGCAATTAATTTGGCCACGCCTAGCATAAATCAGGGGTG - 5`

c) the coding or sense starnd is

DNA: 5` - ATGGAGCCGTTAATTAAACCGGTGCGGATCGTATTTAGTCCCCAC - 3`

d) mRNA: 5`-AUG GAG UCG UUA AUU AAA CCG GUG CGG AUC GUA UUU AGU CCC CAC-3´

Amino acid sequence: Met Glu Ser Leu Ile Lys Pro Val Arg Ile Val Phe Ser Pro His

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