Which restriction enzyme would be used to clone lysozyme coding region? Which in

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Which restriction enzyme would be used to clone lysozyme coding region?

Which insertional marker trait was used to identify colonies with recombinant plasmids?

The size of the recombinant plasmids are__?

The lysozyme coding region can be inserted into the pBNJ vector in two possible orientations. Determine the base pair position (based on the orientation of the insert) of each of the following sites in the recombinant plasmid maps:

Insert Orientation Restriction Enzyme w/in Insert Recombinant Plasmid Position Native HindIII Native BamHI Non-native HindIII Non-native BamHI

Explanation / Answer

1. Abr II is used as restriction enzyme.

2. Amphicilin resistance is used as marker trait. If there is resistance, then insert is placed correctly into the plasmid.

3. Size of recombinant plasmid: 4150 + 515 = 4665 bp approximately.

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Which restriction enzyme would be used to clone lysozyme coding region? Which in

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