Yes, I would use this colony. No, I would not use this colony. the insert was li
ID: 60947 • Letter: Y
Question
Yes, I would use this colony.
No, I would not use this colony.
the insert was ligated into the vector in the native orientation.
the colony did not contain the recombinant plasmid or the original vector.
the insert was ligated into the vector in the non-native orientation.
the insert was not ligated into the the vector.
No, I would not use this colony.
Yes, I would use this colony.
the insert was ligated into the vector in the non-native orientation.
the colony did not contain the recombinant plasmid or the original vector.
the insert was ligated into the vector in the native orientation.
the insert was not ligated into the the vector.
Yes, I would use this colony.
No, I would not use this colony.
the insert was ligated into the vector in the non-native orientation.
the insert was ligated into the vector in the native orientation.
the colony did not contain the recombinant plasmid or the original vector.
the insert was not ligated into the the vector.
Yes, I would use this colony.
No, I would not use this colony.
the colony did not contain the recombinant plasmid or the original vector.
the insert was ligated into the vector in the non-native orientation.
the insert was ligated into the vector in the native orientation.
the insert was not ligated into the the vector.
Colony 1:Yes, I would use this colony.
No, I would not use this colony.
Because the diagnostic gel demonstrated that...the insert was ligated into the vector in the native orientation.
the colony did not contain the recombinant plasmid or the original vector.
the insert was ligated into the vector in the non-native orientation.
the insert was not ligated into the the vector.
Colony 2:No, I would not use this colony.
Yes, I would use this colony.
Because the diagnostic gel demonstrated that...the insert was ligated into the vector in the non-native orientation.
the colony did not contain the recombinant plasmid or the original vector.
the insert was ligated into the vector in the native orientation.
the insert was not ligated into the the vector.
Colony 3:Yes, I would use this colony.
No, I would not use this colony.
Because the diagnostic gel demonstrated that...the insert was ligated into the vector in the non-native orientation.
the insert was ligated into the vector in the native orientation.
the colony did not contain the recombinant plasmid or the original vector.
the insert was not ligated into the the vector.
Colony 4:Yes, I would use this colony.
No, I would not use this colony.
Because the diagnostic gel demonstrated that...the colony did not contain the recombinant plasmid or the original vector.
the insert was ligated into the vector in the non-native orientation.
the insert was ligated into the vector in the native orientation.
the insert was not ligated into the the vector.
Digested with Clal Digested with Xmalll pBNJ Colony1 Colony2 Colony3 Colony4 pBNJ lo Colony2 Colony3 Colon 6236 4540 3256 3905 2621 2621 2621 2621 925Explanation / Answer
Colony 1:
The digestion with ClaI
No, I would not use this colony.the insert was not ligated into the the vector
The digestion with XmaIII:
Yes, I would use this colony
the insert was ligated into the vector in the non-native orientation
Colony 2:
The digestion with ClaI
No, I would not use this colony.Because the diagnostic gel demonstrated that
the insert was not ligated into the the vector
The digestion with XmaIII
No, I would not use this colony Because the diagnostic gel demonstrated that
the insert was ligated into the vector in the non-native orientation
Colony 3:
The digestion with CalI No, I would not use this colony
Because the diagnostic gel demonstrated that the colony did not contain the recombinant plasmid or the original vector
The digestion with XmaIII:No, I would not use this colony.
Because the diagnostic gel states that the colony did not contain the recombinant plasmid or the original vector
Colony 4:
The digestion with CalI: the colony did not contain the recombinant plasmid or the original vector
No, I would not use this colony.Because the diagnostic gel demonstrated that
the insert was not ligated into the the vector
The digestion with XmaIII
No, I would not use this colony Because the diagnostic gel demonstrated that
the insert was ligated into the vector in the non-native orientation
Colony 3:
The digestion with CalI No, I would not use this colony
Because the diagnostic gel demonstrated that the colony did not contain the recombinant plasmid or the original vector
The digestion with XmaIII:No, I would not use this colony.
Because the diagnostic gel states that the colony did not contain the recombinant plasmid or the original vector
Colony 4:
The digestion with CalI: the colony did not contain the recombinant plasmid or the original vector
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