1. What is the importance of micropipettes and gel electrophoresis in genetic en
ID: 59681 • Letter: 1
Question
1. What is the importance of micropipettes and gel electrophoresis in genetic engineering?
2. During the labs, you were often reminded to avoid contact with the pipette tips—for example, you were asked to put the pipette tip on without using your hands, to avoid setting down the micropipette, to use the ejector button to remove the tip, and to keep the tip box closed. If you were working with plasmids and bacterial cells, why would these precautions be important?
3. Study your gel electrophoresis results:
Which solution sample contained a single dye: S1, S2, or S3? How do you know?
The molecular weights for the dyes are 452.38 atomic units (au) for orange G, 669.98 au for bromophenol blue, and 538.62 au for xylene cyanole. How do these weights compare with your original conclusions about the weights of the dyes?
4. Do you think there was a difference in the amount of charge on one of the dye molecules? Explain the reasoning for your response.
Explanation / Answer
1. Importance of micro pipettes and gel electrophoresis in genetic engineering
Micro pipettes are used to handle the very low volumes of DNA/RNA sample and the enzyme used in the reaction.
Gel electrophoresis is used to determine the presence of DNA/RNA its molecular weight, The product of ligation, restriction digestion etc.,
2. These precautions are needed to avoid contamination of DNA from our skin or organisms attached on the hands.
3. The dyes did not travel based on the weight of the molecule, Xylene cyanole [blue] migrated slower than bromophenol blue [purple]. It migrated based on molecular shape and electro negativity
4. Yes there was difference in the amount of charge due to the shape of the molecule.
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