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Bb g Started -2158 x https:// x Prairie View A & M Univer x C WWW sapling learning.com /ibiscms/mod/ibis/view.php?id-1973905 O Not set A 89.3/100 O 0/19/2015 04:01 AM Gradebook Attempts Score Print Calculator Periodic Table Questi 5 of 20 Map 97 An antibody x binds to an antigen Y. When the antigen concentration is 6.0x10 8 M, e is 0.35. 95 What is the dissociation constant, Kd, of the antibody for the antigen? 98 95 Next Give Up & Vi Ch US 20 Copyright 2011 2015 Sapling Learning, Inc. 190 about us careers partners privacy policy terms of use tact us help stidine Search the web and Windows Assignment Information Available From: 8/24/2015 06:00 PM Due Date Not Set Points Possible 100 Grade Category: Graded Description: Policies: Homework n check your answers. You can view solutions when you complete or give up on any question You can keep trying to answer each question until you get it right or give up You lose 5% of the points available to each answer in your question for each incorrect attempt at that O eTextbook OHelp With This Topic Web Help & Videos O Technical Support and Bug Reports 8:10 AM 0/22/2015Explanation / Answer
K d = k d k a = [ Ab ] [ Ag ] [ Ab - Ag ] ( 3 )
where Kd is the dissociation constant, [Ab] and [Ag] are the molar concentrations of unoccupied binding sites on the antibody or antigen respectively, and [Ab-Ag] is the molar concentration of the antibody-antigen complex. The time taken to reach equilibrium is dependent on the rate of diffusion and the affinity of the antibody for the antigen, and can vary widely. The affinity constant for antibody-antigen binding can span a wide range, extending from below 105 mol1 to above 1012 mol1. Affinity constants can be affected by temperature, pH and solvent. Affinity constants can be determined for monoclonal antibodies, but not for polyclonal antibodies, as multiple bonds form between polyclonal antibodies and their antigens. The equilibrium constant of a receptor is a thermodynamic property, and the measured value should be independent of the measurement procedure providing the act of measurement does not damage the receptor, and the experimental conditions (temperature, pH, ionic strength, etc) are maintained.
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