Experiment 7 Gel filtration separates molecules base on: A) Charge B) Affinity C

Question

Experiment 7 Gel filtration separates molecules base on: A) Charge B) Affinity C) Sequence D) Size E) All of the above. 7. 8. During filtration of a protein sample in a high salt solution, the following occurs The protein enters the resin pores and elutes last. A) B) The protein is excluded from the resin pores and elutes first. C) The proteins and salt are mutually exclusive and elute last. The proteins bind the salt and they both eluted first D) E) None of the above The gel filtration gel used in this lab has an exclusion limit of: A) 3 kD 8) 10kD C) 80 kD D) 100 kD E) 150 kD 9. 10. List three important uses of gel filtration in the biochemistry laboratory. 11. A gel filtration column is eluted with three MW standards and an unknown. A plot of the data for the standards is given in the graph below. If the unknown peak elutes at 12.0 ml, what is the molecular mass of the unknown protein? 5.50 5.00 4.50 4.00 3.50 3.00 2.50 2.00 10 20 30 40 Elution volume (ml)

Explanation / Answer

7. D

Gel filteration is also known as Size-exclusion chromatography is a separation technique which is usually applied to large molecules, macromolecular complexes such as proteins and industrial polymers. As the name implies the molecules are separated by their size, and molecular weight.

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