Name: Jonathan mntthys CHM 2290 Gas Chromatography seco Date: 11//5 1. What is c
ID: 587227 • Letter: N
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Name: Jonathan mntthys CHM 2290 Gas Chromatography seco Date: 11//5 1. What is chromatography? 6. What is gas chromatography? In gaschromatography, abbreviated as vaporised so they are in the The se Pe , 4 t ‘ ‘ ^ °· rter ‘t .r i^ ), a mixture of compounds are ^^ enssi^b state. This gas is then pumped through to speed up the rate at which the are separated Gas chromatography can be used to mixture. But another technique is required to help compounds in a the 2. This section of the instrument is called a: 7. This section of the instrument is the Gas Chromatograph Headspace-GC Instrument Diagram 3. This is the inlet, this is where samples that contain 8. Headspace techniques are based on Heary's Lav which states that at a constant temperature, the amount of a given gas dissolved in a given type and volume of liquid is Ditesthy rosturs to the partial pressure of that gas in equilibrium with that liquid. of compounds enter the gas 4. This is the column, this is where the compounds in the mixture are phase is a 5. The column is kept in an The -phase is a gas and the stationary inside the column. 9. The GC in our lab has a Flant Tonized detector in order to keep the analyte in a state 10. Headspace sampling procedures are not affected by un Ve lst lesubstances. 11. a) How many compounds are present in this gas chromatograph? 60 50 40 30 20 10 l can: 11. b) Can you tell from the chromatogram which compound is the most abundant? How would you determine this? Recall the purpose of chromatography Compare the similarities and differences between solid phase extraction and gas chromatography Explain how gas chromatography is used to determine the quantity of a compound in an 10 15 unknown sample. Time I minExplanation / Answer
Chromatography
1. Definition : Separation of mixture of compounds into its individual components using distribution between two phases, one staionary phases and other mobile phase.
2. This section is called gas chromatograph
3. This is inlet where sample that contain mixture of compounds enter the gas chromatograph
4. This is the column, this is where the compounds in the mixture are separated. The mobile phase is a gas and the staionary phase is a solid inside the column
5. The column is kept in an closed container at high temperature to keep the analyte in gas phase.
6. In a gas chromatography, a mixture of compounds are vaporized so they are in the gaseous state. This gas is then pumped through a column, that is at high temperature to speed up the rate at which the components are separated.
Gas chromatography can be used to separate compounds in a mixture.. But another technique is requried to help for detection of compounds.
7. This section of the instrument is called detector unit.
8. Headspace techniques are based on Henry's law, which states that at constant temperature, the amount of a given gas dissolved in a given type and volume of a liquid is directly proprotional to the partial pressure of that gas in equilibrium with the liquid.
9. The GC in the lab has an flame ionization detector (FID).
10. Headspace sampling tecnhique is not affected by non-volatile substances.
11. a) A total of three components are present in the mixture as analyzed by the gas chromatography.
11. b) Looking at the chromatogram given above and calculating the area covered by each peak, we could say that the first peak at 5 min is the major component in the given mixture.
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