Name: Fill in the 5. Here is a serial dilution set up somewhat differently than
ID: 212467 • Letter: N
Question
Name: Fill in the 5. Here is a serial dilution set up somewhat differently than how we set it up in class. blanks and answer the questions below 1 ml 1 ml 1 ml 99 ml dH:O 99 ml dH20 99 ml dH20 Culture (a) After the transfer of culture is made to each bottle, the total volume of each bottle is ml. Show how you calculated this below: ml so this series represents a dilution series. (Hint: The first dilution series we did in class was a 1:10 series; (b) The amount of culture transferred into each bottle is the second was a 1:2 series.) (c) What is the dilution of Bottle A? 1: (d) What is the dilution of Bottle B? 1: (e) What is the dilution of Bottle C? 1: Assume there are 1 billion (1 x 109) CFU/ml in the original culture. How many CFU/ml will be in each bottle after the dilution series is complete? (0) Bottle A (g) Bottle B (h) Bottle C CFU/ml CFU/ml CFU/ml 138Explanation / Answer
Note- in above series each time the dilution will multiply to 100 times.
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