Using the graph on the final page answer the following questions. 25. Estimate K
ID: 58558 • Letter: U
Question
Using the graph on the final page answer the following questions. 25. Estimate Kw and Vida for each set of conditions, units are required. Determine what type of inhibitor molecules A and B are (2pts each). Inhibitor Type Ky or K,M Not Applicable S+A Using the Micheals-Menten equation calculate the fraction of enzyme that has substrate bound to it when the substrate concentration is 324 M, for condition S (5pts) Draw a velocity -vs- substrate plot of the double reciprocal plot. Labeling the axes with correct units is required (5pts).Explanation / Answer
25. Km Vmax
S 5.26mM 0.25min/mol
S+A 5.26mM 0.142min/mol
The molecule A is a non competitive inhibitor
The fraction of enzyme bound to substrate calculated from the graph is 0.125 min/mol
For drawing the double reciprocal graph I need exact values of substrate and its velocity.
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