For a lab we were exploring the concept of chemoselectivity and chemical modific

Question

For a lab we were exploring the concept of chemoselectivity and chemical modification. Using a bovine serum albumin solution and another lysozyme solution were combined with a p-nitrobenzenediazonium solution. Another two test tubes, one with bovine serum albumin and another with a lysozyme solution were also combined with a sulfonic acid derivative. When running these samples through gel electrophoresis only one set of the samples reacted, and it was both of the lysozyme samples, and not the samples containing the bovine serum albumin. Why was this? What made the lysozyme solutions react with sulfonic acid derivative and chemically modify to react with the gel? I am trying to relate the discussion to chemoselectivity.

The p-nitrobenzenediazonium solution was prepared using a solution of p-nitroaniline (0.15 M, 4.5 mol) and an aqueous solution of p-toluenesulfonic acid monohydrate (0.90 M, 13.5 mol).

The sulfonic acid derivative solution was prepared using a solution of 2-amino-5-nitrobenzenesulfonic acid (0.15 M, 4.5 mol) and an aqueous solution of p- toluenesulfonic acid monohydrate (0.90 M, 13.5 mol) and MQ H2O.

Both had an aqueous solution of sodium nitrite (0.5 M, 7.5 mol of NaNO2) added after they were chilled.

Explanation / Answer

lysozyme was azo coupled benzene sulfonic acid and diazonium solutions, and thesecoupling reactions inroduce mainly azo groups to lysyl residues. thats why the test tubes having lysozyme onlyget reacted.

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