Determine the minimum number of theoretical plates, N, required to achieve basel

Question

Determine the minimum number of theoretical plates, N, required to achieve baseline separation with a resolution that is desirable for quantitative analysis of two compounds with an adjusted relative retention, a, of 1.048 and retention factors of 5.58 and 5.85. Incorrect. A minimum desired resolution for quantitative analysis is considered to be 1.50. Use the following relationship to determine the number R-- Number - N- 934 plates of theoretical plates, where R is the resolution, is the adjusted relative retention, and k2 is the retention factor of the later eluting In an attempt to increase the resolution of the separation, a researcher decreased the column length. True or false, decreasing the column length will increase the resolution of the separation? False O True @previous Give Up & View Solution # Try Again O NextExit- | | L

Explanation / Answer

For Number of theoretical plates (N)

Using,

R = (sq.rt.(N)/4)[(alpha-1)/alpha][k2/(1+k2)]

with,

R = 1.5

alpha = 1.048

k2 = 5.85

we get,

1.5 = (sq.rt.(N)/4)[(1.048 - 1)/1.048][5.85/(1+5.85)]

N = 23328 plates

Decreasing the column length will increse the resolution of the separation,

False

Longer run time increases the separation between the peaks and hence resolution increases with column length.

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