In a transduction system using P1 phage, the donor is pur + pro + his - and the

Question

In a transduction system using P1 phage, the donor is pur+ pro+ his- and the recipient is pur- pro- his+. The donor allele pur+ is initially selected after transduction, and 50 pur+ transductants are then scored for the other alleles present. Here are the results:

Genotype

# colonies

pro+ his+

4

pro+ his-

9

pro- his+

24

pro- his-

13

total

50

A)What is the cotransduction frequency for pur and pro?

B)What is the cotransduction frequency for pur and his?

C)Which of the unselected loci is closest to pur?

D)Are pro and his on the same side or opposite sides of pur? Explain.

Genotype

# colonies

pro+ his+

4

pro+ his-

9

pro- his+

24

pro- his-

13

total

50

Explanation / Answer

co tranduction frequency for Pur and pro ;

pro- his- crossed with pro+his- , 4+9 which is equal to 13, This then divided by the total number of offsprings 13/50 which is equal to 0.26

co transduction frquecny for the pur and his recombinants ;

pro+ his - + pro - his + , 24 +4 which is equal to 28 , it is divided by the total number of offsprings which is equal to 28/50 equal to 0.56

Which of the unselected loci is closest to pur?

His loci is closest to the pur in the unselected loci

Are pro and his on the same side or opposite sides of the pur? explain .

pro and his genes are present in the oppsite directions as they are cis acting and produced the recombinants cross over occurs more or less randomly

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