Lab Partner REFLECTIVE EXERCISES Lab Section Date 1 Explain the purpose of mashi
ID: 580178 • Letter: L
Question
Lab Partner REFLECTIVE EXERCISES Lab Section Date 1 Explain the purpose of mashing the strawberry in the bag. 2 Explain at a molecular level how the detergent in the extraction solution weakened the strawberry cell membranes 3 The sodium chloride in the extraction solution serves to separate the DNA from the histones. Based on this information and the structure of DNA, what can you infer about the charge of the histone proteins? Use your inference to explain how the sodium chloride helps separate the DNA and the histones. 4 Why is it important that the ethanol you used in step 10 be ice cold? Lab 20 DNA Extraction 2Explanation / Answer
Answer -1 Why do we “crush” the kiwi/strawberry fruit in the bag?
Crushing the kiwi/strawberry fruit physically breaks apart the cell walls. To break down the cell wall, cellular and nuclear membranes.
Answer -2 Why do we use Detergent?
After the cell walls have been disrupted during mechanical mashing of the fruit, the detergent disrupts the cell and nuclear membranes of each cell to release the DNA. It does this by dissolving lipids and proteins that hold the membranes together.
Answer -4 What does the cold ethanol do?
Everything except the DNA will dissolve in ethanol. The ethanol pulls water from the DNA molecule so that it then collapses in on itself and precipitates. The DNA will become visible as white mucous strands that can be spooled with the wooden applicator stick.
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