1. Use a word from the word bank to answer the following questions. Beer-Lambert
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Question
1. Use a word from the word bank to answer the following questions. Beer-Lambert Law Absorbance Blank Source Absorption spectrum Cuvet Test tube Analyte Calibration curveLambda max Transmission What piece of equipment would you place your sample in to run an absorption spectrum? b. It is the wavelength at which the sample most strongly absorbs. c. In this experiment it was food dyes d. Before taking an absorption spectrum it is important to the spectrophotometer in order to measure the initial intensity of light solution and water from a buret when making solutions for Why was it important to deliver the yellow dye the calibration curve? 2. 3. Identify the name and units for each component of the Beer-Lambert Law.Explanation / Answer
(1)
(a)
The sample is placed is a cuvet.
(b)
Lambda max
(c)
Analyte
(d)
Blank
(3)
In Beer lambert's law, we have:
A = Absorbance, which is unitless
e = Molae absorptivity, having units M-1cm-1
l = path length, in units of cms
C = Conc of analyte solution, in units of M
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