1. Briefly describe what the graph is demonstrating about the enzyme reaction of
ID: 57948 • Letter: 1
Question
1. Briefly describe what the graph is demonstrating about the enzyme reaction of sweet potato at an absorbance of 470 nm.
2. Answer the following according to the graph below:
a. Is the graph for all four samples similar over the full 3 minutes? If not, why do you think this is?
b. The rate of the reaction is determined by the initial part of the reaction where it is linear i.e. the slope of the line. Is the rate of oxidation of guaiacol the same for all of the peroxidase enzymes? If not, what do you think the reason for this could be – there may be several?
Explanation / Answer
1. Spectroscopy is based on the absorbance of the sample solution which depends on colour intensity. As the reaction proceeds, colour will be developed that can be read at certain absorbance. From the graph, we can say that the absorbance is increasing with time, means the reaction is progressing.
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