1. Briefly describe what the graph is demonstrating about the enzyme reaction of
ID: 57666 • Letter: 1
Question
1. Briefly describe what the graph is demonstrating about the enzyme reaction of sweet potato at an absorbance of 470 nm.
2. Answer the following according to the graph below:
a. Is the graph for all four samples similar over the full 3 minutes? If not, why do you think this is?
b. The rate of the reaction is determined by the initial part of the reaction where it is linear i.e. the slope of the line. Is the rate of oxidation of guaiacol the same for all of the peroxidase enzymes? If not, what do you think the reason for this could be – there may be several?
3. Determine the peroxidase activity in each of the plant tissues in “?moles guaiacol oxidized per gram fresh weight per minute” [i.e. ?moles.g-1.fresh wt.min].
Start 00:00:00 End 00:03:00 2.5 1.5 0.5 00:00:00 0.0.50 00:00:50 00:01:40 --A470 Elapsed Time (HH:MM:SS) 00:02:30Explanation / Answer
1. The graph shows the rates of free radical formation for substrate. The maximum wavelength for the rate of formation is 470 nm. The change in the absorbance at this wavelength is demonstrated in the graph.
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