Select the research techniques that can be used to study the GC content, genome
ID: 57076 • Letter: S
Question
Select the research techniques that can be used to study the GC content, genome size, and the number of chromosomes comprising the bacterial genome. sedimentation-velocity centrifugation fluorescent in situ hybridization (FISH) DNA microarray analysis polyacrylamide gel electrophoresis (PAGE) density gradient centrifugation pulse-field gel electrophoresis (PFGE) The bacterium is found to carry a virus that may play a role in the disease. Initial genetic characterization has determined the base composition, or relative percentage of nucleotides, in the viral genome. Select the questions that can be answered using this information. What is the genetic material of the virus? How large is the viral genome? Does the genome have repetitive sequences? Does the virus have multiple chromosomes? How is the viral genome replicated? Is the genome single or double stranded?Explanation / Answer
Research method
Description
Pulsed-field gel electrophoresis
DNA molecules move through a gel with an electrical field, in which the voltage is constantly switched in three directions. PFGE separates DNA ranging in size from kilobases to megabases.
Sedimentation-velocity centrifugation
UV absorption is used to observe movement of DNA during centrifugation to determine the velocity of sedimentation of the DNA sample. Mass and shape determine movement of the molecule, allowing molecular weight calculations.
Density gradient centrifugation
DNA molecules are centrifuged on a CsCl gradient, separating the molecules by density and allowing characterization of DNA composition.
PFGE, sedimentation-velocity centrifugation, density gradient centrifugation.
2nd part: What is the genetic material of the virus? Is the genome single or double stranded?
Research method
Description
Pulsed-field gel electrophoresis
DNA molecules move through a gel with an electrical field, in which the voltage is constantly switched in three directions. PFGE separates DNA ranging in size from kilobases to megabases.
Sedimentation-velocity centrifugation
UV absorption is used to observe movement of DNA during centrifugation to determine the velocity of sedimentation of the DNA sample. Mass and shape determine movement of the molecule, allowing molecular weight calculations.
Density gradient centrifugation
DNA molecules are centrifuged on a CsCl gradient, separating the molecules by density and allowing characterization of DNA composition.
Related Questions
drjack9650@gmail.com
Navigate
Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.