1.) a) Following partial purification of ACTase, a 10 mg/ml aliquot of enzyme co

Question

1.) a) Following partial purification of ACTase, a 10 mg/ml aliquot of enzyme contains 15 units of enzyme activity under optimal conditions (pH = 7).   Given a one ml sample of enzyme, how many umoles of aspartate will be converted to carbamoyl aspartate in 30 seconds under optimal conditions?

b) What is the specific activity in 1 ml of the enzyme preparation?

c) What will happen to specific activity if the enzyme is fully purified?                                                      

d) If the ACTase enzyme is 50% pure, what will be the turnover number at a SINGLE catalytic site? (assume a molecular weight of 180,000 Daltons for the entire ACTase enzyme)

(please explain)

Explanation / Answer

b. Formula for specific activity:

SA=U/mg

U= units

Mg concentration of the enzyme

= 15/10

= 1.5

Specific activity in the 1 ml of the enzyme preparation is 1.5.

c. If the enzyme is fully purified, then the activity of the enzyme increases.

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