Scientists have engineered bacteria to produce human proteins such as human grow

Question

Scientists have engineered bacteria to produce human proteins such as human growth hormone (hGH) to help treat dwarfism. Suppose the unmodified eukaryotic gene coding for hGH is inserted directly into a bacterial chromosome. Select the reasons why no expression would be seen in the bacterial cell for the hGH gene. The gene will induce attenuation in the bacterial cell, which will cause a delay in the transcription and translation of the hGH gene. Bacteria cannot remove intronic sequence from a gene, so if the gene for hGH were transcribed, it would translate to a nonfunctional protein. The bacterial RNA polymerase will not bind to the eukaryotic promoter sequence. The bacterial nucleoid does not have the proper proteins to transcribe the eukaryotic gene.

Explanation / Answer

Order the steps that would be used in a laboratory to engineer a bacterium that could express the human gene coding for hGH.

Step 1: Isolate the human gene that produces hGH

Step 2: The hGH protein is isolated from tissues

Step 3: Transform the vector into an E,coli.bacterium

Step 4: Generate cDNA of the hGH gene using reverse transcriptase

Step 5: Insert the hGH cDNA into a bacterial vector near a promoter site.

Extra Step : Isolate the mDNA of the hGH gene

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