Scientists have engineered bacteria to produce human proteins such as insulin to
ID: 256752 • Letter: S
Question
Scientists have engineered bacteria to produce human proteins such as insulin to help treat diabetes. Suppose the unmodified eukaryotic gene coding for insulin is inserted directly into a bacterial chromosome. Select the reasons why no expression would be seen in the bacterial cell for the insulin gene.
Bacteria cannot remove intronic sequence from a gene, so if the gene for insulin were transcribed, it would translate to a nonfunctional protein.
The gene will induce attenuation in the bacterial cell, which will cause a delay in the transcription and translation of the insulin gene.
The bacterial nucleoid does not have the proper proteins to transcribe the eukaryotic gene.
The bacterial RNA polymerase will not bind to the eukaryotic promoter sequence.
Explanation / Answer
The first reason is bacteria do not have the process called splicing hence no introns will be removed resulting in the formation of the truncated protein..
Secondly the bacteria RNA polymerase will not recognize the eukaryotic ribosome binding site hence the polymerase will not be able to bind and no transcription... As bacteria RNA polymerase recognize Shine Dalgano sequence whereas the eukaryotic organism has Kozak sequence whre RNA polymerase binds...!!
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