You collected data in the form of time to starch disappearance. However, enzyme

Question

  

You collected data in the form of time to starch disappearance. However, enzyme activity is usually quoted as a rate. Convert your collected data to rate form (mg of substrate consumed / unit time), and produce a graph of this data (rate vs. substrate concentration): hint: you must first convert the starch volumes to amounts using the C1V1 = C2V2 equation, and convert 1% starch in to lg/ 100mL equivalent,

Use Appendix 1 for hints using EXCEL

Which quantity, rate or substrate concentration, should be on which axis?

What trend does your data show, and does this support or fail to support the hypothesis you propose in #1, above?

Tube # 1% Strach pH 6.8 buffer Distilled water 0.1% amylase Time to starch disappearance 1 0 mL 2 mL 2 mL 1 mL 30 2 0.5 mL 2 mL 1.5 mL 1 mL 300 3 1.0 mL 2 mL 1.0 mL 1 mL 360 4 1.5 mL 2 mL 0.5 mL 1 mL 8:03 5 2.0 mL 2 mL 0 mL 1 mL 11

Explanation / Answer

The rate steadily increases when more substrate is added because more of the active sites of the enzyme are being used which results in more reactions so the required amount of oxygen is made more quickly. Once the amount of substrate molecules added exceeds the number of active sites available then the rate of reaction will no longer go up. This is because the maximum number of reactions are being done at once so any extra substrate molecules have to wait until some of the active sites become available.

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