1.The PCR (polymerase chain reaction) protocol that is currently used in laborat

Question

1.The PCR (polymerase chain reaction) protocol that is currently used in laboratories was facilitated by the discovery of a bacterium called Thermus aquaticus in a hot spring inside Yellowstone National Park, in Wyoming. This organism contains a heat-stable form of DNA polymerase known as Taq polymerase, which continues to function even after it has been heated to 95°C.

a.Why would such a heat-stable polymerase be beneficial in PCR?

b.What would happen if it weren’t heat stable?

c.How might you choose a region of DNA for a PCR primer so as to increase the temperature necessary for primer annealing (to minimize nonspecific PCR products)?

d.A PCR reaction begins with 5 double stranded segment of DNA. Estimate the number of double-stranded copies of DNA that are present after the completion of 15 amplification cycles?

2. Based on the following replication bubble, label W, X, Y, and Z as leading or lagging strands.

Explanation / Answer

Answer 1

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