QUESTION 12 Match the following structural and functional attributes of a -indep

Question

QUESTION 12

Match the following structural and functional attributes of a -independent transcription termination signal with the corresponding sequences on the DNA template strand.

causing the RNA polymerase to pause

directs the formation of hairpin in RNA

promotes the dissociation of the RNA-DNA hybrid

oligo(U) stretch in RNA

A-rich region

GC-rich palindromic region

3.5 points   

QUESTION 13

The aim of the experiment was to understand the function of the enzyme -gal.

The aim of the experiment was to identify new antibacterial interventions by interfering with transcription.

The aim of the experiment was to identify what is the consensus sequence of the -10 box and what is the consensus spacer length.

The aim of the experiment was to test the effects on promoter activity of lac promoter variants that were made to progressively look more and more like the canonical bacterial promoter in their -10 box sequence and consensus spacer length.

3.5 points   

QUESTION 14

One of the benefits of transcription and mRNA (as opposed to having the translation machinery works directly on the DNA) is signal amplification. The term here means that

the "message" encoded in one gene is "broadcasted" many times - first in the form of many copies of mRNA transcribed from the gene and then as many copies of protein molecules translated from the mRNA.

mistakes in the DNA (that is mutations) can be amplified to create a real problem, which will lead to reduction in fitness and fewer offspring.  

signals conveyed to the cell from the outside can get to the nucleus.

only the amplitude of gene expression needs to be regulated, but not its frequency.

      -       A.       B.   

causing the RNA polymerase to pause

Read Answer Items for Question 12       -       A.       B.   

directs the formation of hairpin in RNA

Read Answer Items for Question 12       -       A.       B.   

promotes the dissociation of the RNA-DNA hybrid

Read Answer Items for Question 12       -       A.       B.   

oligo(U) stretch in RNA

Read Answer Items for Question 12 Answer A.

A-rich region

B.

GC-rich palindromic region

Explanation / Answer

Question 12

causing the RNA polymerase to pause

directs the formation of hairpin in RNA

promotes the dissociation of the RNA-DNA hybrid

oligo(U) stretch in RNA

Question 13: The question is unclear as the picture/table is missing, however going through the data the option is:

D: The aim of the experiment was to test the effects on promoter activity of lac promoter variants that were made to progressively look more and more like the canonical bacterial promoter in their -10 box sequence and consensus spacer length.

Question 14:

The correct option is

A: the "message" encoded in one gene is "broadcasted" many times - first in the form of many copies of mRNA transcribed from the gene and then as many copies of protein molecules translated from the mRNA.

GC-rich palindromic region

causing the RNA polymerase to pause

     GC-rich palindromic region    

directs the formation of hairpin in RNA

      A-rich region    

promotes the dissociation of the RNA-DNA hybrid

      A-rich region   

oligo(U) stretch in RNA

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