QUESTION 12 1.00000 points The ?Ge of reaction A- B is +2 kcal/mole. The ?Go of

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QUESTION 12 1.00000 points The ?Ge of reaction A- B is +2 kcal/mole. The ?Go of reaction B-+ C is .9 kcal/mole. If these two reactions are coupled, the AG of A B will... O increase. O decrease. O stay the same. QUESTION 13 1.00000 points Sa Consider the Lineweaver-Burke equation shown below. What is the Vmax for this equation? *Do not include units with your answer** 0 131.0.1957 -0.5 1+0.1667 QUESTION 14 1.00000 points Save A Imagine that you have a box containing 200 quarters. 100 of them are heads up and the other 100 are tails up, what is the ?G and ?G° for this scenario?

Explanation / Answer

12. Here, the standard free energy change of A----B stay the same. When we are coupling this two chemical reaction overall free energy change will be additive of free energy of individual step.

A------B. 2 kcal/mol

B--------C. -9kcal/mol

A-------C. -7kcal/mol

Here, B is comman in both reaction and standard free energy change of overall reaction is thermodynamically favourable. Here reaction 2 is thermodynamically favourable which drive thermodynamically unfavourable reaction A----B. Therefore, coupling doesnt impact standard free energy change of indiviual reaction.

13. lineweaver burk plot is reciprocal of michellis menton equation. Michellis menton equation is

v= Vmax S/Km + S

1/v = Km+S/Vmax S

1/v = Km/Vmax S + S/ Vmax S

1/v = Km/Vmax 1/S + 1/Vmax

So here the equation is given

1/Vmax = 0.1667

Vmax = 5.998

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