Procedure: Part 1- Tuning the Instrument Wavelength 1) Prepare 6 calibration sol
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Procedure: Part 1- Tuning the Instrument Wavelength 1) Prepare 6 calibration solutions nmediumtesttubes using 1 and 5 mlgraduatedpipettes.- Calibration Solution 0.00025M Bromothymol Blue (mL) 0.010 M HCI (mL) 4.00 4.30 4.50 1.00 0.70 ymol Blue (mL) 0.010 M NaOH (mL) C4 C5 C6 0.00025M Bromoth 1.00 0.70 0.50 4.00 4.30 4.50 (2) Prepare 5 standard solutions in medium test tubes using 1 and 5 ml graduated pipettes. 0.10M KH2PO: (mL) 0.10M 0.00025M Bromothymol blue (mL) Solution S7 S8 S9 S10 S11 1.00 1.00 1.00 1.00 1.00 K2HPO4 (mL) 4.00 3.00 2.00 1.00 0.00 0.00 1.00 2.00 3.00 4.00 AL..alirle nrinninle and illn.+ H2O = HsO+ + in-, show the 'shift, when you have the BTBExplanation / Answer
We shall use the dilution equation. The dilution equation is
M1*V1 = M2*V2 where M1 = concentration of BTB in the stock; V1 = volume of stock BTB added; M2 = concentration of BTB in the diluted solution and V2 = final volume of the solution.
We have V2 = 5.00 mL in C1-C6.
Consider C1 as an example. We have M1 = 0.00025 M, V1 = 1.00 mL and V2 = 5.00 mL. Plug in the dilution equation to obtain M2.
(1.00 mL)*(0.00025 M) = (5.00 mL)*M2
====> M2 = (1.00*0.00025)/(5.00) M = 5.0*10-5 M
The concentration of BTB in C1 is 5.0*10-5 M. Use the table below to find out the concentrations of BTB in C1-C3.
Calibration Solution
Volume of 0.00025 M BTB added (mL)
Final volume of calibration solution (mL)
Concentration of BTB in the calibration solution (M)
C1
1.00
5.00
5.0*10-5
C2
0.70
5.00
3.5*10-5
C3
0.50
5.00
2.5*10-5
Use the dilution equation again to find the concentration of BTB in C4. We have M1 = 0.00025 M, V1 = 1.00 mL and V2 = 5.00 mL. Plug in the dilution equation to obtain M2.
(1.00 mL)*(0.00025 M) = (5.00 mL)*M2
====> M2 = (1.00*0.00025)/(5.00) M = 5.0*10-5 M
The concentration of BTB in C4 is 5.0*10-5 M. Use the table below to find out the concentrations of BTB in C4-C6.
Calibration Solution
Volume of 0.00025 M BTB added (mL)
Final volume of calibration solution (mL)
Concentration of BTB in the calibration solution (M)
C4
1.00
5.00
5.0*10-5
C5
0.70
5.00
3.5*10-5
C6
0.50
5.00
2.5*10-5
Calibration Solution
Volume of 0.00025 M BTB added (mL)
Final volume of calibration solution (mL)
Concentration of BTB in the calibration solution (M)
C1
1.00
5.00
5.0*10-5
C2
0.70
5.00
3.5*10-5
C3
0.50
5.00
2.5*10-5
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