You have completed the sequencing of many parts of the dystrophin gene. Which of

Question

You have completed the sequencing of many parts of the dystrophin gene. Which of the following pieces can NOT be included in the final contig? (Note that some sequences were sequenced on the forward strand and others on the reverse strand).

AATGGTGTCCCCA---(>750bp)-------TGTTCGTTGAAAAAAGTTTGTC
GTGTATGTGTAGTGT ---(>750bp)--- AAGTGTAGTGCCCCGTGTGACGTGTA
GACAAACTTT --- (>750bp)----------AGTTTGTCACCAAGTGTGTAACA
GAGTGTGTAGTTT---(>750bp)--------AAAAGTGTGACTGTGACCCGTGTGTA
TACACAGGGTCTC---(>750bp)-------TGTACCCCAGTGTCGATCTTGTACGTG
TGTTACACACTTG --- (>750bp)---------TTAGTTTGTTTGAGTGTGTAGTTT

Piece 1

Piece 2

Piece 4

Piece 5

Explanation / Answer

Piece 2

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