PLEASE DONT JUST POST very techincal information, need simplified response and e

Question

PLEASE DONT JUST POST very techincal information, need simplified response and explanation that is clear.

You have isolated a unique mammalian protein, alpha, that recruits other partners in its normal function in the cell. Using a yeast two-hybrid system you have identified a potential binding partner, beta, for your bait protein. Assuming that you could express and purify both components, describe TWO different experimental methodologies (including SALIENT details) that you would conduct to determine whether the two proteins interact in vivo in a mammalian cell line?

Explanation / Answer

A green flourescent protein can be tagged to alpha - beta protein hybrid and their interaction can be detected with flourescent microscopy which reveals in which cells they are acting individually or combindly and what is role of these proteins in cellular metabolism and cellular response to these proteins can be studied.

DNA micro array prepared along with the new alpha - beta protein hybrid and the interaction of these proteins with cells in mammalian cell line can be studied.

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