I’m so confused with this hw can someone please explain in detail with the steps

Question

I’m so confused with this hw can someone please explain in detail with the steps and have the units :/ concentration of 33*10-6 M. Graph the data using excel and calculate the values for Km and Vmax. Make sure you show the equation for the curve obtained and used. 0.20 0.26 0.33 1.00 1.43 1.67 2.08 4.44 3.- (1 point) Using the data obtained in exercise 2, calculate Kcat for the enzyme 4.- (1 point) Using Lineweaver-Burk, you determined an inhibitor to cause a mixed inhibition when assayed at a concentration of 5 mM. If the equation that characterized the reaction without inhibitor is y-0.6030.1978 (units y=1/(mM/min) and x=mM), the reaction in the presence of the inhibtor y 2.45020.3514. Calculate the values for and 5.- (1 point) Using Lineweaver-Burk, you determined an inhibitor to cause a mixed inhibition when assayed at a concentration of 5 mM. If the equation that characterized the reaction without inhibitor is y 0.6030.1978 (units y-1/(mM/min) and x-mM), the reaction in the presence of the inhibitor y 2.45020.3514. Calculate the values ki and ki

Explanation / Answer

A non competitive inhibitor will be no match against the substrate. that means it will be atached in the enzyme in a differente site instead the substrate that will be in the active site. So the affinity will be no affected in the non competitive inhibitor but it will reduce the velocity in which the reaction happens.

Tocalculate the Km and Vmax we will need ti plot the 1/v against 1/S values. With this plot we can calculate this values with this equations.

V=Vmax([S]/Km+[S])

And

1/V=Km/Vmax(1/[S])+!/Vmax.

So with the values and the equation we calculate Km and Vmax.

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