Spectrophotometric Analysis gs Review View Tell me what you want to do 1 Normal

Question

Spectrophotometric Analysis gs Review View Tell me what you want to do 1 Normal 1No Sp. Heding Heding2 Title Subtitle Subrle Em.. Emphasis Intense l Paragraph Syles Name: Date: Section: Pre lab 7: Analysis of Food Dyes 1· (20 points) Select from the following words and complete the statement below 1. Molarity 2. Excel 4. Beer's Lavw 5. Control Objective 6. Quantitative The of Allura red in Koolhid_, In order to achieve thi data that will allow for the calculation of the will be applied to create a standard curve, using DI as of this lab is to use a spectrophotometer to obtain -'----will be used to organize and manipulate experimental data. 2 (10 points) Using the color wheel and electromagnetic spectrum given in the adjacent figure, I predict Allura Red will display a the most absorption at b. Primarily transmit nm range colored light Onange 3. 120 points) Explain how is the standard curve used to interpolate the concentration of an unknown sample of Allura red?

Explanation / Answer

1. The objective of this lab is to use spectrophotometer to obtain quantitative data that will allow for the calculation of the molarity of Allura red in Kool-Aid. In order to achieve this, Beer's law will be applied to create a standard curve, using DI as a control. Excel will be used to organize and manipulate experimental data.

Using the color wheel and electromagnetic spectrum given in the adjacent figure.

2.I predict Allura Red will display

a) the most absorption at 500-520 nm range.

b) Primarily transmit red colored light.

Explanation: The observed color is complementary to the color of light absorbed. Red color is complementary to green color. Thus, Allura Red absorbs most wavelengths in the 500-520 nm range. Wavelengths of 640-700 nm are transmitted by Allura red, thus it appear red.

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