Spectrophotometer assays are used for Sample purification Sample identification

Question

Spectrophotometer assays are used for Sample purification Sample identification Sample quantification a and b and c lambda max of each compound is The wavelength at which the compound has minimum absorption The wavelength at which the compound has maximum absorption The wavelength at which the compound shows no transmit ion The wavelength at which the compound shows 100 % transmit ion A subunit of catalase enzyme has a molecular weight of 60,000 D and hemoglobin 68,000, which protein will elute first from a saphead G-75 column (fractionation range of, 3000-80,000 D) Catalase subunit Hemoglobin Both proteins elute together proteins will not be fractionated by this G-75 column in all type of chromatography, the mobile phase may be Solid Gas Liquid a and c b and c in all type of chromatography, the stationary phase may be Liquid Gas Solid b and c a and c The total volume of the solvent and adsorbing materials (stationary phase) in a packed column is Void volume Bed volume Elution volume Exclusion limit Affinity chromatography can be applied to isolate biological macromolecules Study the molecules interaction

Explanation / Answer

8. Option c is correct. It is used for both identification and quantification.

9. Option a, i.e, wavelength at which compound has maximum absorption.

10. Option a, catalase subunit will come out first.

11. Option e, it can be gas or liquid.

12. Option e, it can be solid or liquid.

14. Study the molecular interaction.

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