Lab Report for Experiment: Separation of Plant Pigments by Chromatography Descri

Question

Lab Report for Experiment: Separation of Plant Pigments by Chromatography Describe the fractions collected during the column chromatography experiment: Fractions Color of Fraction Based on your observations during the column chromatography experiment, which is the more polar substance? Chlorophylls Carotenes Sketch the TLC chromatogram(s) below. Indicate what each original spot was and show final spots on the chromatogram. If your TLC experiment was not a success then sketch what it should have looked like based on the results of the column chromatography experiment.

Explanation / Answer

Answers:

1. phases are two types one is stationary phase and second is the mobile phase.

2.TLC stands for Thin layer chromotography.

3. yes, must and should be first run less polar solvent in the column because separation is clear if you want the run more polar solvent first time then separation is not clear and mixture of compound is obtained.

4.a) If let us the solvent run upto 1 cm and spot B is 0.8 cm and spot A is 0.6 cm then Rf of B is 0.8/1.0=0.8 cm. Rf of A is 0.6/1.0=0.6 cm.

4.b) low Rf value us more polar so spot A is the more polar.

4.C) spot B is the eluting first and collect first it because it has less polar than spot A.

4.D) the ratio of acetone and hexane should be decreased because Rf value is 0.3 means it is very non polar spot so decrease the polarity.

5.1. only fraction 1 give the pure sample of A.

5.2. fractions 3,4 and 5 combined and give the pure sample of B.

5.3. fractions 7&8 combined and give the pyre sample of C.

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