Date Partner Name 1. How is column chromatography related to TLC? Gas chromatogr

Question

Date Partner Name 1. How is column chromatography related to TLC? Gas chromatography? Be sure to discuss mobile phase, stationary phase, sample size, sample type in your answer 2. How is Rf in TLC related to elution order in column chromatography? products of this reaction. Explain based on their structures. 3. What is the elution order of the 3.In this lab, why was the solvent changed in the middle of the column chromatography procedure? 4. When a mixture is to be separated by column chromatography, why is it better to switch from a less-polar to a more-polar eluent rather than the reverse order? 5. What effect will the following factors have on a chromatographic separation: a) too polar of an adsorbent; b) Collection of large fractions;

Explanation / Answer

ANSWER 1

TLC

Stationary phase = coated on aluminium plate

mobile phase = liquid for moves from the bottom of the plate

sample size = small

sample type = detecting the composition of the sample

COLUMN CHROMATOGRAPHY

Stationary phase = packed in vertical glass tube

mobile phase = liquid moves from top to the bottom

sample size = large

sample type = seperating the mixture of compounds

GAS CHROMATOGRAPHY Stationary phase = liquid form

mobile phase = inert gases helium or nitrogen etc

sample size = both small and large

sample type = identification and quantifying given volatile organic

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