CHIP followed by DNA sequencing determined that the Hif1a recognition motif for
ID: 54246 • Letter: C
Question
CHIP followed by DNA sequencing determined that the Hif1a recognition motif for the growth factor VEGF gene to is GCGTG.
Which of the following is true about an EMSA assay?
Addition of Hif1a with radioactively labeled GCGTG will retard/slow the migration of GCGTG compared to GCGTG alone
Addition of Hif1a with radioactively labeled GCGTG will speed the migration of GCGTG compared to GCGTG alone
Addition of VEGF with radioactively labeled GCGTG will speed the migration of GCGTG compared to GCGTG alone
Addition of VEGF with radioactively labeled GCGTG will retard/slow the migration of GCGTG compared to GCGTG alone
Addition of Hif1a with radioactively labeled GCGTG will retard/slow the migration of GCGTG compared to GCGTG alone
Addition of Hif1a with radioactively labeled GCGTG will speed the migration of GCGTG compared to GCGTG alone
Addition of VEGF with radioactively labeled GCGTG will speed the migration of GCGTG compared to GCGTG alone
Addition of VEGF with radioactively labeled GCGTG will retard/slow the migration of GCGTG compared to GCGTG alone
Explanation / Answer
EMSA stands for electrophoretic mobility shift assay. It is a technique based on the observation that protein:DNA complexes migrate more slowly than free linear DNA fragments when subjected to non-denaturing polyacrylamide or agarose gel electrophoresis [1,2]. Since the rate of DNA migration is retarded or shifted when bound to protein, the assay is otherwise called a gel shift or gel retardation assay.
Hence, the correct statement is (a) - Addition of Hif1a with radioactively labeled GCGTG will retard/slow the migration of GCGTG compared to GCGTG alone.
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