DILUTION TECHNIQUES AND CALCULATIONS Twofol d dilutions are PROCEDURE etsseru te
ID: 541564 • Letter: D
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DILUTION TECHNIQUES AND CALCULATIONS Twofol d dilutions are PROCEDURE etsseru temonly usod to dilutepa mula applies: a dilution of 100 ofsample into 1001 . The same fr, of saline equals blank a. If the dilution is into a tube, mix the contents 100 ,which is 2 -2 on a vortex mixer or by rolling the tube back 100 +100 200 2 and forth between your hands. b. If the dilution is into a 99-ml blank, hold the cap in place with your index finger and shake If 100 of this 12 dilution is put in 100-1 of saline, the final dilution is the bottle up and down through a 35-em are. 2. It is neceasary to use a freah pipette for each dilu tion in a series, but it is permissible to use the same pipette to remove several samples from the same bottle, as when plating out samples from a series of dilutions. PROBLEMS Practice caleulating sorial dilutione using tho following problems 1 m 1 ml 9 ml 9 ml 9 mi 1. Dilution: 1 ml 1 mi 99 ml 99 ml 9 ml 9 ml 2. Diluton:Explanation / Answer
1. sample = 1 ml after addition in 1st test tube total volume = 1+9 = 10 ml, dilution factor 1/10
2nd test tube 1 ml of 1/10 dilution factor in 9 ml solution, then dilution factor will be 1/10* 1/10 = 1/100
similarly, 3rd test tube it will be 1/100 *1/10 = 1/1000
2. sample = 1 ml after 1st container total volume = 99+1 = 100 ml, dilution factor = 1/100
2nd: 1/100 * 1/100 = 1/10000
3rd: 1/10000 * 1/10 = 1/100000
4th : 1/100000 * 1/10 = 1/1000000
4. 1st: 50/ (50+50) = 1/2
2nd: 1/2*50/ (50+50) = 1/4
3rd: 1/4*50/ (50+50) = 1/8
4th: 1/8*50/ (50+50) = 1/16
5th: 1/16*50/ (50+50) = 1/32
5. 1st: 0.1 gm sample in 0.9 ml = 1/9 gm /ml
2nd: total volume 0.1+0.9 = 1ml, dilution factor = 0.1/1 = 1/10
3rd: 1/10*1/10 = 1/100
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