DILUTION WORKSHEET #02 Original SampleTube 01 Tube 02 Tube 03 Tube 04 1:100 14 1
ID: 217185 • Letter: D
Question
DILUTION WORKSHEET #02 Original SampleTube 01 Tube 02 Tube 03 Tube 04 1:100 14 13 15 Each lat ube contains 0 nL of solution 1 mL 1 mL 1 ml 1 mL Carpet 119 colonies 29 colonies No colonies all math work must be showrn 1. What volume of the original sample and what volume of H20 would you use to create Tube 01? 2. What volume of the Tube 01 solution and what volume of H20 would you use to create Tube 02? 3. What volume of the Tube 02 solution and what volume of H20 would you use to create Tube 03? 4. What volume of the Tube 03 solution and what volume of H20 would you use to create Tube 04? plate would you use the colony count to calculate sample density and why? 6. What is the density of the Tube 01? (give answer in CFU/mL) 7. What is the density of the Tube 02? (give answer in CFU/ml) 8. What is the density of the Tube 03? (give answer in CFU/mL) 9. What is the density of the Tube 04? (give answer in CFU/mL) 10. What is the density of the original sample? (give answer in CFU/mL)Explanation / Answer
1) The volume of original sample is 100 µl and H2O is 9.90 ml would be used to create Tube 01 (It is 1:100 times dilution of the original sample).
2) The volume of Tube 01 sample is 2 ml and H2O is 8 ml would be used to create Tube 02 (It is 1:4 times dilution of the Tube 01).
3) The volume of Tube 02 sample is 2.5 ml and H2O is 7.5 ml would be used to create Tube 03 (It is 1:3 times dilution of the Tube 02).
4) The volume of Tube 03 sample is 1.67 ml and H2O is 8.33 ml would be used to create Tube 04 (It is 1:5 times dilution of the Tube 03).
5) I will use Tube 02 for the colony count because it has a countable number and it is easy to calculate.
6) The density of Tube 01 is 119 CFU/ml.
7) The density of Tube 02 is 29 CFU/ml.
8) The density of Tube 03 is 10 CFU/ml.
9) The density of Tube 04 is (10 / 5) = 2 CFU/ml.
10) If we consider, the density of Tube 02 is 29 CFU/ml.
So, in Tube 01 will be (29 X4) = 116 CFU/ml.
Original sample is 100 times of the Tube 01.
So, the density of the original sample is 11600 CFU/ml that means 1.16 X 104 CFU/ml.
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