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1. The chemical \"label\" that permits visualiztion of DNAfragments in sequencin

ID: 5415 • Letter: 1

Question

1. The chemical "label" that permits visualiztion of DNAfragments in sequencing machines is a. 32P b. 35S c. tritium d. fluorescent dyes e. none of the above 2. Bacteria protect their own DNA from restriction enzymedamage by adding ----- groups to certain nucleotides. a. carboxyl b. methyl c. amino d. hydroxyl e. phosphate 3. A polylinker region in a cloning plasmid is characterizedby a. multiple expression vectors b. multiple restriction cut sites c. multiple cloning targets d. multiple methyl groups e. multiple reporter genes 1. The chemical "label" that permits visualiztion of DNAfragments in sequencing machines is a. 32P b. 35S c. tritium d. fluorescent dyes e. none of the above 2. Bacteria protect their own DNA from restriction enzymedamage by adding ----- groups to certain nucleotides. a. carboxyl b. methyl c. amino d. hydroxyl e. phosphate 3. A polylinker region in a cloning plasmid is characterizedby a. multiple expression vectors b. multiple restriction cut sites c. multiple cloning targets d. multiple methyl groups e. multiple reporter genes

Explanation / Answer


For question number 2, the answer in methyl groups, Thebacteria methylate the regions of their DNA, which are cut by theirrestriction endonucleases as to not destroy their own DNA.
And as for question #3 i am not sure. Sorry