18.01 1. Match the following 1. Provide the relative locations of genes on a chr
ID: 53850 • Letter: 1
Question
18.01
1.
Match the following
1. Provide the relative locations of genes on a chromosome based on recombination frequency.
2. Constructed using landmarks in a DNA sequence, such as restriction enzyme sites, or the DNA.
3. A small stretch of DNA that occurs only one time in the genome.
4. A continuous piece of DNA, established by putting cut pieces of DNA back together based on size and overlap.
5. Hybridization of fluorescently labeled cloned DNA to whole chromosomes.
6. Specific recognizable sequences in the genome, such as restriction sites, STSs, or ESTs.
A. genetic linkage maps
B. fluorescent in situ hybridization (FISH)
C. contig
D. physical maps
E. sequenced-tagged sites (STSs)
F. landmarks
2.
How is a physical map different from a genetic map?
A. a physical map can be confirmed by STSs.
B. a physical map will reveal the order of genes on a chromosome.
C. a physical map is measured by recombination rate.
D. a physical map can be confirmed by FISH.
E. a physical map is measured in base pairs.
3.
Distances between landmarks in physical maps are measured in kbs (kilobases), which are _____ base pairs.
A. 100
B. 1000
C. 10
D. 500
E. 50
4.
In the construction of physical maps, the technique of _____ is used to separate restriction digested or PCR generated DNA fragments.
A. gel electrophoresis
B. thin-layer chromatography
C. mass spectrophotometry
D. sedimentation
E. centrifugation
5.
Short Tandem Repeats (STR loci) are alleles that
A. have known sequences that can be PCR amplified.
B. are 1 cM unit apart on the chromosome.
C. always occur in the same frequency between unrelated individuals.
D. commonly influence the linkage map of a genome.
18.02
6.
Match the following
1. The first generation of vectors designed to allow larger pieces of DNA to be cloned, but that proved to allow rearrangements or even to lose portions of cloned DNA.
2. Vectors made in E. coli that accept inserts between 100 and 200kb long, maintained as a single copy.
3. A sequence that is consistent across all copies of multiple different sequenced clones.
A. bacterial artificial chromosomes (BACs)
B. consensus sequence
C. yeast artificial chromosomes (YACs)
7.
Artificial chromosomes are used as
A. cDNA sources.
B. contigs.
C. vectors.
D. selectors.
E. sequencers.
8.
The artificial chromosome most commonly used now is
A. SSR.
B. EST.
C. YAC.
D. STS.
E. BAC.
9.
The difference between clone by clone sequencing and shotgun sequencing is
A. Bioinformatics can be used for clone by clone sequencing, but not for shotgun cloning.
B. clone by clone sequencing uses small randomly cut pieces of DNA, and uses a computer to put together the overlaps.
C. clone by clone sequencing aligns large contigs of DNA.
D. clone by clone sequencing is faster and more accurate than shotgun sequencing.
10.
Haemophilus influenzae was the first organism to have its entire genome sequenced. Which approach was used to sequence its genome?
A. mapping with transposition.
B. reverse transcription.
C. clone-by clone sequencing.
D. shotgun DNA sequencing.
11.
In automated sequencing a fluorescently labeled dideoxynucleotide is added to the 5' end of a strand of DNA.
True
False
12.
In the Sanger method of sequencing, the incorporation of dideoxynucleotides act as chain terminators because they do not have a
A. 2-H.
B. 2-OH.
C. 5-PO4.
D. 3-H.
E. 3-OH.
Explanation / Answer
1. Match the following ;
answer ;option 1 -A, option 2 -D, option 3 -E, Option 4-C , option 5 - B , option 6 - F
2. how physical mapping and genetic mapping is diffrence
answer; option E - physichal map measured in base pairs
3. Distances between landmarks in physical maps are measured in kbs (kilobases), which are _____ base pairs.
answer ; option B 1000 base pairs .
4.In the construction of physical maps, the technique of _____ is used to separate restriction digested or PCR generated DNA fragments.
answer ; option A - gel electrophoresis .
5.Short Tandem Repeats (STR loci) are alleles that
answer ; option c
6.
answer ; option 1 -c, option 2 -A , option 3 - B
7. option C -Vectors
8. option C - YAC
9. option B - clone by clone sequencing uses small randomly cut pieces of DNA, and uses a computer to put together the overlaps.
10. option D short gun sequencing
11.TRUE -In automated sequencing a fluorescently labeled dideoxynucleotide is added to the 5' end of a strand of DNA.
12. option E - 3 prime hydoxyl group .
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