You are trying to purify each of the proteins in a mixture of three proteins lis

ID: 53755 • Letter: Y

Question

You are trying to purify each of the proteins in a mixture of three proteins listed below:

Name MW (kDa) pI

A 30 3.8

B 30 9.5

C 150 6.8

a. Which protein can be purified by gel filtration chromatography on a Superdex200 column? (2 pt)

b. Which protein will bind to a CM-cellulose column running at pH 6.0? (2 pts)

c. Which protein can be effectively purified from ion exchange chromatography on a DEAE-cellulose column running at pH 6.8? (2 pts)

d. Design an effective strategy to purify each of these three proteins from the mixture. (3 pts)

e. After you purify all the proteins, how can you check whether they are pure or not? (1 pt)

a) It can separate all three proteins because its range is 10 - 600 kDa.

b) Protein C will bind, as its pH range is 5 - 9.

c) Same as above, Protein C.

e) By running the separate samples on gel electrophoresis, it will tell they are pure or not. Single band will be observed for each pure protein.

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