BCA Assay Results Analysis You have just calculated the amount of GFPS65T (in mg

Question

BCA Assay Results Analysis

You have just calculated the amount of GFPS65T (in mg) recovered by student Alex. Do you expect this to be less, more, or the same as the mg amount of GST-GFPS65T in the initial lysate sample (before purification commenced). Your answer should include a consideration of milligrams of protein and moles of protein.
Answer less, more, or same, then justify your answer briefly. This section should be no more than 1/3 page.

6 (1 mark) 3 (1 mark) 4 (1 mark) Dil'n of Ass for GFPS65T1 in diluted Actual GFP565 in vol. of the otal mg of sample each sample (ug/ Find the undiluted GFP S65T GFP S65T in sample this by solving the egn, sample (m eluate (ml) entire sample 0.751 0.238 0.754 0.251 0.115 113 64.2 0.131 0.076 27.2 0.136 Amount of GFP565T in the sample (mg) (averaged from samples) (1 mark) This m ndiluted SECTION 9: CONCLUSION 2 You have just calculated the amount of GFP565T (in mg) recovered by student Alex. Do you expect this to be less, more, or the same as the m mount of GST-GFPS65T in the initial lysate sample (before purification commenced). Your answer should include a consideration of milligrams of protein and moles of protein. Answer less, more, or same, then justify your answer briefly. This section should be no more than 1/3 page. (2 marks total)

Explanation / Answer

Hfr cells involved in conjugation transfer host genes in a linear fashion. The genes transferred depend on both the Hfr strain and the length of time during which the transfer occurred. Therefore, a population containing several different Hfr strains will appear to have an almost random transfer of host genes. This is similar to generalized transduction, in which the viral protein coat forms around a specific amount of DNA rather than specific genes. In generalized transduction, any gene can be transferred.

F´ factors arise from improper excision of an Hfr from the bacterial chromosome. They can have only specific bacterial genes on them because the integration site is fixed for each strain. Specialized transduction resembles this in that the viral particle integrates into a specific region of the bacterial chromosome and then, upon improper excision

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