BCA Assay of GFPS65T eluate justification (Mostly question B) 2.5 0.0011x R2 0.9

Question

BCA Assay of GFPS65T eluate justification (Mostly question B)

2.5 0.0011x R2 0.9887 1.5 B 1 0.5 2000 2500 1000 1500 BCA Concentration (ug/mL) a) Comment on the quality of your standard curve (poor/good). In one sentence, justify your answer. The quality of the standard curve is good based on the high R value 0.9887 b) Comment on the accuracy of the quantification of protein in the GFps65T eluate, and its correlation with the spectrophotometric determination. In two sentences, explain your answer.

Explanation / Answer

b) Samples that are assayed must be relevant, although you can observe some differences
   because of varied quantities of protein or some other errors they should be relatable in the sense
   they should be comparable. The above mentioned reason can be the reason accounting for
little inaccuracy.

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