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Please also tell me why you picked the your answer. Show any sources used. Which

ID: 528979 • Letter: P

Question

Please also tell me why you picked the your answer. Show any sources used.

Which ONE of the following statements is CORRECT in relation to the distribution ratio in Liquid Extraction. Distribution Ratio = Conc. of solute in organic phase/Conc. of solute in aqueous phase Distribution Ratio = Conc. of solute in aqueous phase/Conc. of solute in organic phase Distribution Ratio = Conc. of Silica/Conc. of Methanol Distribution Ratio = Mobile phase/Stationary phase Fatty acids in biological fluids will undergo chemical derivatisation using a catalyst, in an esterification reaction to form which ONE of the following compounds? Aldols Carboxylic acids Fatty acid methyl esters Ketols A Diode Array detector (DAD) in HPLC analysis is beneficial for which ONE of the following reasons? It allows for a single wavelength to be selected for detection It allows a wide range of wavelengths to be detected. The detector can be used with gradient elution and isocratic elution systems It allows high concentrations of analytes to be detected. Which ONE of the following statements describes how separation of polar and non-polar analytes occurs within normal phase chromatography in HPLC. Polar analytes have an affinity for the non-polar stationary phase, this slows down the separation as they spend more time in the column and elute out first Non-Polar analytes have an affinity for the polar mobile phase, therefore they move the fastest and are eluted first. Non-polar analytes have an affinity for the non-polar mobile phase, therefore they move the fastest and elute out first. Polar analytes have an affinity for the polar stationary phase, therefore they move the slowest and are eluted first.

Explanation / Answer

Ans 10- Distribution Ratio (Kd) = Conc. of solute in organic phase

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Conc. of solute in water (aq. phase)

Distribution ration is given as Kd which is concentration of solute in two different solvent.

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