1.This analyte reacts with 2-oxoglutarate + NADPH in the presence of GLDH to for

Question

1.This analyte reacts with 2-oxoglutarate + NADPH in the presence of GLDH to form glutamate + NADP + H2O at 37ºC. A decrease in absorbance is measured at 340 nm due to the formation of NADP from NADPH. This describes the principle of the analysis of:

alkaline phosphatase.

alanine transaminase.

ammonia.

glutamine

2.

Which of the following photodetectors is the most sensitive?

Barrier layer cells

Phototubes

Photomultiplier tubes

Null-point potentiometers

3.

Nephelometric measurements are based upon

Increase of light energy after antigen-immunoglobulin complexes are formed in solution

Decrease of light energy monitored after antigen-immunoglobulin complexes are formed in solution

The difference in energy in which a molecule will absorb and emit a photon

The absorbance of characteristic photons by immunoglobulin-antigen complexes0

4.

A turbidimetric method that is used for the quantitation of total protein in urine and CSF specimens uses the reagent:

biuret.

bromcresol green.

phenol.

sulfosalicylic acid.

5.

A patient with Biliary obstruction has a serum bilirubin assay performed. The results determined using the Jendrassik-Grof method are as follows:
Total bilirubin:0.8mg/dL
Conjugated bilirubin:1.0mg/dL
You do not report the results. The results obtained are most consistent with:

A technical error occurring during analysis

Insufficient accelerator added to the total bilirubin reaction

A reduced reaction time for the conjugated bilirubin reaction

Excess Diazo reagent added to the conjugated bilirubin reaction

alkaline phosphatase.

alanine transaminase.

ammonia.

glutamine

Explanation / Answer

1. Answer is b. alanine transminase(ALT)

ALT is an intracellular cytoplasmic enzyme. It is widely present in body tissue, with maximum in liver and kidney. It is a key enzyme in gluconeogenesis, which catalyzes the transfer of amino groups from alanine to 2-oxoglutarate.

The ALT reaction involves an amino acid and an oxoacid both as substrate and products. Quantitation makes use of the formation of the oxoacid (pyruvate), using lactate dehydrogenase to reduce it with NADH to form lactate. Thereaction is followed by measuring the decrease in absorbance at 340n.m.

Alanine + 2oxoglutarate (ALT) pyruvate + glutamate

Pyruvate + NADH + H+ (LD) lactate + NAD+

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