You need to prepare 250. mL of a 0.700 M aqueous solution of sucrose, C_12H_22O_
ID: 525749 • Letter: Y
Question
You need to prepare 250. mL of a 0.700 M aqueous solution of sucrose, C_12H_22O_11(aq), which is used frequently in biological experiments. Which type of glassware should you use to make this solution (assuming that the accuracy of the concentration is important)? A B C How should the correct amount of solute be obtained? Measure out x mol of solid sucrose on a molemeter. Measure out x cm of sucrose with a ruler. Measure out x g of solid sucrose on a balance. Now that you have selected the correct unit above, what is the value of x? How should the solute and solvent be mixed in the container? Add the solute, then fill the container to the 250-mL mark with water. Mix a small amount of water with the solute to dissolve it, then fill with water to the 250-mL mark. Fill the container with water to the 250-mL mark, then add the solute.Explanation / Answer
Which type of glassware should be used to make this solution?
Answer: C
This is a volumetric flask these are used to make the precise dilutions & standard solution in the biological experiments.
How should the correct amount of solute be obtained?
Answer:- measure out x g of solid sucrose on a balance.
Sucrose is a solid & the solids can be accurately measured on the analytical balance. Calculate the amount of sucrose required to make 0.700 M, 250 ML aqueous solution on the balance.
What is the value of x?
Answer = 59.90 g
342.29 g of sucrose dissolved in 1000 mL makes the 1M solution.
0.34229 g in 1mL makes the 1M solution
0.34229 x 0.7 = 0.2396 g in 1mL makes the 1M solution
0.2396x 250 = 59.90 g in 250 mL of water makes the 0.700 M solution
Alternatively one can use the formula to calculate
C = m/v x 1/MW
How should the solute & solvent be mixed in the container?
ANSWER: Mix a small amount of water with the solute to dissolve it, and then fill with the water to the 250 Ml mark.
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