You want to separate a mixture of benzene, toluene and p-xylene isocratically by

Question

You want to separate a mixture of benzene, toluene and p-xylene isocratically by using reverse phase chromatography but don't get a baseline separation between the analytes with the acetonitrile/water mobile phase that you are using. What will you do? (a) Decrease the acetonitrile content of your mobile phase. (b) Change to wider column. (c) Change to a shorter column. (d) Increase the acetonitrile content of your mobile phase. When the particle diameter of the stationary phase decreases, keeping all other physical characteristics of the column equal, it will have the following effect: Which of the following statements is incorrect? (a) The pressure in the system increases. (b) The mass transfer to the stationary phase is facilitated. (c) The retention factor increases. (d) The peak width decreases. Which of the following is the most popular detector for hydrocarbons in GC? (a) fluorescence (b) flame ionization detection (c) electron capture detector (d) conductivity detector

Explanation / Answer

18) As all three are aprotic in nature, on increasing the acetonitrile content of mobile phase makes the compinds to separate smothly.

19) As the particle diameter of stationary phase decreases, the pressure of the system will decrease(due to decrease in the particle size will decrease the intramolecular molecular distance).

20) FID(flame ionization detection) method will the foremost useful detector for hydrocarbons in GC.

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