I need a pre lab for plasmid purification using alkaline lysis Pre-Lab: (5 point

Question

I need a pre lab for plasmid purification using alkaline lysis Pre-Lab: (5 points) What scientific concept(s) will be learned in this lab activity? a. First identify the concept(s) (principle, theory, law) emphasized in the lab exercise b. What laboratory techniques will be learned in this activity? Look through the introduction and the protocol itself. What are you doing? Gel electrophoresis, column chromatography? Spectrophotography? c. What is the objective (or goal) for the lab? An objective is an activity you will be performing. This answer should be written in sentence form. It should use 1. Example A: In this lab we will test the quantity of chemical X present in the sample. 2. Example B: In this lab we will quantitate the binding of a protein to a ligand. 3. Example C: In this lab, we will purify DNA from an onion Points will be based on the quality of the response. Responses should be well-thought out and demonstrate logical thinking skills. St tudents should show an understanding of what is to be discovered or acco:mplished by completing the lab exercise Materials and Methods: (2 points except as noted below) Refer the reader to the source of the protocol. For example, if the protocol you followed t is to be discovered or accomplished by completing the lab exercise. vas in the lab ma nual, reference the lab manual. If the protocol was handed out in class, ist the source as Dr. Pattison's esource as Dr. Pattison's BCHS3201 protocol. If you deviate from the published naterials or method in any way, be sure to note the change in this section. This may rovide useful information in explaining the results of the experiment later in the sion section. Another experimenter should be able to replicate the experiment g nothing but your materials and methods section and referenced protocol for uidance. Quantities, times, and temperatures should he includad in al

Explanation / Answer

a. The scientific concepts that can be learned from this lab activity are as follows:

b. The techniques that can be learned are as follows:

c. Purification of plasmid DNA from bacteria.

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