.22 is wrong You have discovered a novel protein that has a p = 3.3. o study the
ID: 52327 • Letter: #
Question
.22 is wrong
You have discovered a novel protein that has a p = 3.3. o study the functional properties o this new protein, your research group has made a mutant that contains two amino acid charges-namely, a surface Phe residue in the normal protein has been replaced by His (side chain plc = 6.1), and a surface Gln has been replaced by Gln (side chain pKa = 6.0) Part A Is the pI of the mutant protein predicted to be greater than, less than, or the same as the pI of the normal protein? The pI of the mutant protein predicted to be greater than the pI of the normal protein. The pI of the mutant protein predicted to be less than the pI of the normal protein. The pI of the mutant protein predicted to be the same as the pI of the normal protein. Correct Part B Support your answer with the calculation of an overall charge on the mutant at pH = 5.5. Express your answer using three decimal places.Explanation / Answer
part B ;
pI = 1/2 [pKa 1+Pkb 2} ,pka 1= 6.1,pkb =6.0
= 1/2 [6.1+6.0] =6.15
sp pI of the muatant at ph 5.5 is 6.15 as histidine is weakly basic and polar and glutamate is acidic and polar .so the overall net charge is weakly acidic . so there will be no net charge on the mutant protein
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