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For the reaction: (NH2)2CO --------> (NH4)2 CO3 ----------> 2NH3 + CO2 +H20 The

ID: 521422 • Letter: F

Question

For the reaction: (NH2)2CO --------> (NH4)2 CO3 ----------> 2NH3 + CO2 +H20

The standard required 20 mL of 0.01N NaOH to titrate the HClThe 1 mL sample required 10 mL of 0.01N NaOH to titrate the HClWhat was the concentration of NH3 in the sample (mg/mL)?, and what was the original concentration of urea if the urine sample was diluted 10 times? Given: Mw Urea: 60.0 g/mol; Mw NH3 =17.01 g/mol ; Stoichiometry: (1 Urea/2 NH3)!     

a) 1.7 mg/mL, 60 mg/mL

b) 1.7 mg/mL, 30 mg/mL

c) 17 mg/mL, 60 mg/mL

d) 17 mg/mL, 30 mg/mL

Explanation / Answer

for the given reaction,

moles of NaOH used = moles of HCl = moles of NH3

moles of NaOH = 0.01 M x 10 ml = 0.1 mmol for 1 ml sample

concentration of NH3 in the sample = 0.1 mmol x 17.01 g/mol/1 ml

                                                         = 1.7 mg/ml

when the urine sample is diluted 10 times,

concentration of NH3 = 1.7 x 10 = 17 mg

moles NH3 = 17/17 = 1 mmol

original concentration of urea = 1 mmol x 60 = 60 mg/ml

Answer : a) 1.7 mg/ml, 60 mg/ml

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