The PCR (polymerase chain reaction) protocol that is currently used in laborator

Question

The PCR (polymerase chain reaction) protocol that is currently used in laboratories was facilitated by the discovery of a bacterium called Thermus aquaticus in a hot spring inside Yellowstone National Park, in Wyoming. This organism contains a heat-stable form of DNA polymerase known as Taq polymerase, which continues to function even after it has been heated to 95 degree C. Why would such a heat-stable polymerase be beneficial in PCR? A) Each cycle includes a "hot" denaturation phase (95 degree C), which allows the primers to the target DNA. B) Each cycle includes a "hot" denaturation phase (95 degree C), which servers to sterilize the culture. C) Each cycle includes a "hot" denaturation phase (95 degree C), which activates the Taq polymerase. D) Each cycle includes a "hot" denaturation phase (95 degree C), which separates the hydrogen bonds that hold the strands of the template DNA together. E) More that one of the above are correct. In the context of molecular genetics, reverse transcription PCR (RT-PCR) refers to _____ A) assembling a DNA sequence from an mRNA B) assembling an RNA sequence from a DNA sequence C) translating in the 3' to 5' direction D) transcribing first, then translating E) making an amino acid sequence from a DNA sequence Which below is not one of the requirements for PCR (Polymerase Chain Reaction)? A) two primers B) DNA polymerase like Taq polymerase C) dNTPs D) Ca^2+ Which is the correct order for the three steps of PCR? A) Primer annealing rightarrow Denaturation rightarrow Extension B) Denaturation rightarrow Primer annealing rightarrow Extension C) Extension rightarrow Primer annealing rightarrow Denaturation D) Denaturation rightarrow Extension rightarrow Primer annealing Suppose the 3 temperatures for the 3 steps of PCR are 65 degree C, 72 degree C, 94 degree C. What temperature setting below for PCR is correct? A) Primer annealing (72 degree C) B) Denaturation (94 degree C) C) Extension (65 degree C)

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