When you make up 500 ml of acetate buffer, which of the following best describes

Question

When you make up 500 ml of acetate buffer, which of the following best describes the steps you will take Selected Use the Henderson-Hasselbalch equation to work out the ration of acetic acid to sodium acetate required, split the Answer: total moles of buffer into that ration, convert moles to grams, add to 500 ml of ddH20, check the pH with pH meter and adjust if necessary with acetic acid or sodium acetate. Use the Henderson-Hasselbalch equation to work out the moles of acetic acid and sodium acetate required, add to 500 ml of ddH20, check the pH with pH meter and adjust if necessary with HCl or NaOH. Use the Henderson-Hasselbalch equation to work out the moles of acetic acid and sodium acetate required, add to 500 ml of ddH20, check the pH with pH meter and adjust if necessary with acetic acid or sodium acetate. Use the Henderson-Hasselbalch equation to work out the moles of acetic acid and sodium acetate required, add to 400 ml of ddH20, check the pH with pH meter and adjust if necessary with HCI or NaOH. Use the Henderson-Hasselbalch equation to work out the ration of acetic acid to sodium acetate required, split the total moles of buffer into that ration, convert moles to grams, add to 500 ml of ddH20, check the pH with pH meter and adjust if necessary with HCl or NaOH. Use the Henderson-Hasselbalch equation to work out the ration of acetic acid to sodium acetate required, split the total moles of buffer into that ration, convert moles to grams, add to 500 ml of ddH20, check the pH with pH meter and adjust if necessary with acetic acid or sodium acetate.

Explanation / Answer

Use the Henderson-Hasselbalch equation to work out the ratio of acetic acid to sodium acetate required, spilt the total moles of buffer into that ratio, convert moles to grams, add to 500 mL of ddH2O, check the pH with pH meter and adjust if necessary with HCl or NaOH.

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