how to isolate the human clone? 7. (10 points) You have created a CDNA library o
ID: 51788 • Letter: H
Question
how to isolate the human clone?
7. (10 points) You have created a CDNA library of human DNA from fibroblasts (a type of skin cell), and have obtained from a colleague a cloned histone H4 gene from yeast. (A cDNA library is essentially a collection of mRNAs from an organism, but in the form of DNA rather than RNA.) Note that the sequence of histone H4 is highly conserved among eukaryotes. How would you use your library and cloned yeast gene to isolate the human H4 cDNA done? how to isolate the human clone?Explanation / Answer
Answer:
Cloning is the process in which, the desired of the interest is selected to produced more number of identical copies.
The steps involve in the cloning are
1) Isolate of the geneof interest by action of restriction endonucelases-II (R.E=II). R.E cuts the DNA genome into the small fragments besides the gene of the interest.
2) Selection of the appropiate vector in necessary, once vector is choosen, then gene of interest should be introduced into the vector. In this case Yeast vector called YAC, is generally used for eukaryotic genes. as human DNA is eukaryotic DNA, we can prefer Yeast vector.
3) Next step is to insert into the Host, cloniing vector with gene of the interest should grow in the host, generally E.Coil , a bacteria used as a host due to its short generation time of 20min.
4) Next step is isolation of the recombinant, cloned cells are isolated by extracting DNA. Other molecular biology techniqes such as Blotting, are used to identify. Sequenceing of the genome can be done from cloned DNA.
5) Check for matching using Bioinformatics tools
Related Questions
Navigate
Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.